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. 2016 Jan 6;12(1):e1005362. doi: 10.1371/journal.ppat.1005362

Fig 5. Measuring the affinity of the F258L and wt JCV OBDs to bind to the central region of the core origin via ITC.

Fig 5

A. The four pentanucleotide containing Site II based duplex oligonucleotide, derived from the JCV origin of DNA replication, used in these studies. The GAGGC sequences are underlined. B. An ITC titration for the wt JCV T-ag OBD with DNA. C. An ITC titration for the JCV T-ag OBD F258L mutant with DNA. For each study, the calorimetric trace is shown in the top panel; the X-axis is time in minutes, while the Y-axis is power in ucal/s. The binding isotherms are shown in the bottom panel; the X-axis is the molar ratio of the indicated OBD to DNA. The Y-axis is kcal/mol of OBD. Values for KD and stoichiometry (N) are indicated.