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Mean observed heterozygosity for 14 microsatellite loci of field and colony Anopheles darlingi by collection date or colony generation. A statistically significant difference was detected among collections (repeated measures analysis of variance [ANOVA], P = 0.00068) with a significant Bonferroni-corrected post hoc pairwise t tests between May 2012 specimen and both F5 and F21 generations (P = 0.0245 and 0.0093, respectively). Error bars represent one standard error (SE).
