Table 1.

Number of Anopheles darlingi (N), alleles (A), private alleles (A_P), alleles with specified allelic frequencies, inbreeding coefficient (F_IS), and Wilcoxon signed rank and mode shift tests for bottlenecks using 14 microsatellite loci, by source population

	Field An. darlingi			Colony An. darlingi
	May 12	October 12	April 13	F5	F12	F21
N	53	41	12	21	28	30
An.s.	113	104	80	72	73	62
AP*	12	6	4	5	4	1
A frequency ≥ 0.50*	2†	4	3	7	7	9†
A frequency < 0.05*	48†‡	34§	19†	25	19	10‡§
FISn.s.	0.47	0.48	0.52	0.61	0.57	0.63
Wilcoxon test
TPM∥n.s.	0.0083	0.0083	0.0148	0.0676	0.1206	0.1206
SMMn.s.	0.9973	0.9877	0.8662	0.9979	0.9324	0.8794
Mode shift	Normal	Normal	Normal	Normal	Normal	Normal

ANOVA = analysis of variance; SMM = stepwise mutation model; TPM = two-phase model.

^*Statistically significant differences by collection, over all 14 loci in A_P, A frequency ≥ 0.50, and A frequency < 0.05 (repeated measures ANOVA, P = 0.012, P = 0.000821, and P = 9.23 × 10⁻⁶, respectively).

^{†, ‡, §}Statistically significant post hoc, Bonferroni-corrected pairwise t tests.

^∥TPM settings: proportion of SMM in TPM = 0; variance of geometric distribution for TPM = 36 [per Bottleneck software website (http://www1.montpellier.inra.fr/CBGP/software/Bottleneck/pub.html)].

^n.s.No statistically significant differences by collection, over all 14 loci in A, nor F_IS (ANOVA, P = 0.0669, and P = 0.9, respectively).