. 2015 Nov 4;93(5):998–1001. doi: 10.4269/ajtmh.15-0336

Table 1.

Number of Anopheles darlingi (N), alleles (A), private alleles (A_P), alleles with specified allelic frequencies, inbreeding coefficient (F_IS), and Wilcoxon signed rank and mode shift tests for bottlenecks using 14 microsatellite loci, by source population

	Field An. darlingi			Colony An. darlingi
	May 12	October 12	April 13	F₅	F₁₂	F₂₁
N	53	41	12	21	28	30
A^n.s.	113	104	80	72	73	62
A_P^*	12	6	4	5	4	1
A frequency ≥ 0.50^*	2^†	4	3	7	7	9^†
A frequency < 0.05^*	48^†^‡	34^§	19^†	25	19	10^‡^§
F_IS^n.s.	0.47	0.48	0.52	0.61	0.57	0.63
Wilcoxon test
TPM^∥^n.s.	0.0083	0.0083	0.0148	0.0676	0.1206	0.1206
SMM^n.s.	0.9973	0.9877	0.8662	0.9979	0.9324	0.8794
Mode shift	Normal	Normal	Normal	Normal	Normal	Normal

ANOVA = analysis of variance; SMM = stepwise mutation model; TPM = two-phase model.

Statistically significant differences by collection, over all 14 loci in A_P, A frequency ≥ 0.50, and A frequency < 0.05 (repeated measures ANOVA, P = 0.012, P = 0.000821, and P = 9.23 × 10⁻⁶, respectively).

^{†, ‡, §}

Statistically significant post hoc, Bonferroni-corrected pairwise t tests.

^∥

TPM settings: proportion of SMM in TPM = 0; variance of geometric distribution for TPM = 36 [per Bottleneck software website (http://www1.montpellier.inra.fr/CBGP/software/Bottleneck/pub.html)].

^{^n.s.}

No statistically significant differences by collection, over all 14 loci in A, nor F_IS (ANOVA, P = 0.0669, and P = 0.9, respectively).