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Simulations (blue = SimFonia; green = EasySpin) of the first derivative EPR spectra (red) of [14N]-NH2Y730• and [15N]-NH2Y730• in H2O assay buffer. (A) [14N]-NH2Y730• at 94 GHz. (B) [14N]-NH2Y730• at 9 GHz. (C) [15N]-NH2Y730• at 9 GHz. See Table 1 for parameters. The simulations with EasySpin appear slightly broader due to the effect of forbidden transitions.
