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. Author manuscript; available in PMC: 2016 Jan 6.
Published in final edited form as: Mol Cell. 2015 Mar 19;57(6):1110–1123. doi: 10.1016/j.molcel.2015.02.016

Figure 3. Mutational analysis of the L. lactis yybP-ykoY riboswitch.

Figure 3

A) SHAPE with and without 0.3 mM Mn2+ present, on wild-type and mutant L. lactis yybP-ykoY aptamer domains. B) IVT assays on binding site mutants in a range of Mn2+ conditions. C) Overall view of the metal docking region of the A41U mutant crystal structure (colored) overlaid with the wild-type structure (gray). D) Close-up view of the A41U mutant binding site. Sr2+ are shown in green. The 2Fo−Fc electron density is in teal, contoured to 2.7 σ, showing a water (red) bound to MB.