Fig. 3.

The levels of phosphorylated MAPK and PI3K signaling proteins treated with triclosan. Western blot analyses were done to measure the protein levels of signaling molecules related to MAPK and PI3K after 1 or 3 h treatment with 50 μM triclosan in cultured rat NSCs. All experiments were individually performed during the 4^th day of NSC culture in vitro (DIV 4). The results are shown as the percentage relative to the vehicle. Values are expressed as the mean ± S.E.M. ^*p<0.05, ^**p<0.01, and ^***p<0.001 vs. vehicle.