Table 2. Effect of moderate drought and T. evansi infestation on plant defense proteins in tomato leaves at 4 and 10 days post infestation.
Non-infested | Infested | ANOVA (p<0.05) | |||
---|---|---|---|---|---|
Control | Drought | Control | Drought | ||
4 Days Post Infestation | |||||
Protease inhibitors (% Inhibition) | |||||
Papain | 48±2ab | 64±2a | 31±5c | 45±5b | D;I |
Cathepsin B | 49±3 | 62±6 | 63±8 | 66±4 | - |
Trypsin | 40±8 | 44±2 | 49±8 | 43±2 | - |
Chymotrypsin | 61±7a | 45±6b | 64±2a | 63±4a | I |
Oxidative enzymes (specific activity) | |||||
Polyphenol oxidases1 | 1.1±0.1b | 2.1±0.1a | 2.5±0.2a | 2.6±0.3a | D;I;D*I |
Peroxidases2 | 2.7±0.2b | 5±0.5a | 6.8±1.1a | 5.1±0.2a | I; D*I |
10 Days Post Infestation | |||||
Protease inhibitors (%Inhibition) | |||||
Papain | 57±3c | 81±3a | 71±4b | 86±3a | D;I |
Cathepsin B | 40±5c | 60±7b | 63±6b | 83±5a | D;I |
Trypsin | 37±3ab | 54±4a | 31±8b | 52±5a | D |
Chymotrypsin | 69±4 | 65±6 | 74±6 | 77±4 | - |
Oxidative enzymes (specific activity) | |||||
Polyphenol oxidases1 | 2.2±0.1b | 2.5±0.4b | 3.7±0.5a | 3±0.4ab | I |
Peroxidases2 | 4.3±0.5b | 4±0.5b | 8.1±0.9a | 5.8±0.6b | D; I |
(1) PPO: nmol hydrolyzed Cathecol/ mg Protein*min
(2) POD: nmol hydrolyzed Guaiacol/ mg Protein*min.
Data are mean ± SE. D (drought) and I (mites infestation) indicate significant factors in the Two-Way ANOVA. Different lower case letters within rows indicates significant differences (Newman-Keuls test at p<0.05).