| Strains |
|
|
|
E. coli
|
|
|
| DH5α |
Φ80 lacZΔM15 Δ (lacZYA-argF) U169 hsdR17 recA1endA1 thi-1 |
Lab collection |
| SM10 |
F-
thi-1 thr-1 leuB6 recA tonA21 lacY1 supE44(MuC+) λ- Kmr
|
Lab collection |
|
P. aeruginosa
|
|
|
| PAO1 |
Phenazine-1-carboxylic acid and its derivatives producer, Wild type, AprSpr
|
Lab collection |
| Δphz1
|
phz1 locus deleted and inserted with aacC1, SprGmr
|
This study |
| Δphz2
|
phz2 locus deleted and inserted with aph, SprKmr
|
This study |
| Δphz1phz2
|
phz1 deleted and inserted with aacC1, phz2 deleted and inserted with aph, simultaneously, GmrKmr
|
This study |
| ΔphzA2Z
|
the partial phzA2B2 deleted and chromosomally fused with the truncated lacZ in frame, Spr
|
This study |
| Δphz1phzA2Z
|
phz1deleted and inserted with aacC1 in the mutant ΔphzA2Z, SprGmr
|
This study |
| ΔphzA1Z
|
the partial phzA1B1 deleted and chromosomally fused with the truncated lacZ in frame, Spr
|
This study |
| ΔphzA1Zphz2
|
phz2 deleted and inserted with aph in the mutant ΔphzA1Z, SprKmr
|
This study |
| Plasmids |
|
|
| pBluescript II SK |
Clone vector, ColE, Apr
|
Stratagene |
| pGEM-T |
T-vector, ColE, Apr
|
Promega |
| pEX18Tc |
Gene replacement vector with MCS from pUC18, oriT+ sacB+, Tcr
|
[39] |
| pEXZ1 |
pEX18Tc containing a 2.0-kb phz1-flanking PCR fragment, Tcr
|
This study |
| pEXZ1G |
A 2.0-kb phz1-flanking PCR fragment inserted with aacC1 in pEX18Tc, TcrGmr
|
This study |
| pEXZ1Z |
A 2.4-kb phzA1B1-deleted PCR fragment cloned in pEX18Tc, Tcr
|
This study |
| pEXZ1Zlac |
A 2.4-kb phzA1B1-deleted PCR fragment fused in frame with the truncated lacZ in pEX18Tc, Tcr
|
This study |
| pEXZ2 |
pEX18Tc containing a 3.0-kb phz2-flanking PCR fragment, Tcr
|
This study |
| pEXZ2K |
A 3.0-kb phz1-flanking PCR fragment inserted with aph in pEX18Tc, TcrKmr
|
This study |
| pEXZ2Z |
A 2.5-kb phzA2B2-deleted PCR fragment cloned in pEX18Tc, Tcr
|
This study |
| pEXZ2Zlac |
A 2.5-kb phzA2B2-deleted PCR fragment fused in frame with the truncated lacZ in pEX18Tc, Tcr
|
This study |
| pME10Z1 |
pME6010 containing a 6.9-kb phz1 cluster, Tcr
|
This study |
| pME10Z2 |
pME6010 containing a 6.8-kb phz2 cluster, Tcr
|
This study |
| pME15Z1 |
A 0.9-kb phz1 upstream fragment and a translational phzA1′-′ lacZ fusion with first 8 phzA1 codons in pME6015, Tcr
|
This study |
| pME15Z2 |
A 0.9-kb phz2 upstream fragment and a translational phzA2′-′ lacZ fusion with first 8 phzA2 codons in pME6015, Tcr
|
This study |
| pME22Z1 |
pME6522 carrying a 902-bp upstream region of phz1 (from -902 to +1) and transcriptional fusion phz1-lacZ, Tcr
|
This study |
| pME22Z2 |
pME6522 carrying a 517-bp upstream region of phz2 (from -517 to +1) and transcriptional fusion phz2 -lacZ, Tcr
|
This study |
| pME6010 |
Low capy vector in Pseudomonas sp., Tcr
|
[43] |
| pME6015 |
pVS1-p15A shuttle vector for translational lacZ fusion, Tcr
|
[43] |
| pME6522 |
pVS1-p15A shuttle vector for transcriptional lacZ fusion and promoter probing, Tcr
|
[44] |
| pNM481 |
′lacZ fusion vector, Apr
|
[45] |
| pNM482 |
′lacZ fusion vector, Apr
|
[45] |
| pUC18-19Km |
ColE, aph, kanamycin resistance cassette flanked with multiple restriction sites, AprKmr
|
[42] |
| pUCGm |
ColE, aacC, gentamycin resistance cassette flanked with multiple restriction sites, AprGmr
|
[40] |
