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CD46 expression in RPE grown on RPE-derived ECM. Expression of ZO-1 at the cell margins of ARPE-19 cells (apical intercellular area) (A). There is abundant expression of CD46 intracellularly and at the basal margins of the cell (B), DAPI nuclear staining (C), and merged image (D). XZ projection shows apical expression of ZO-1 and basolateral and intracellular localization of CD46 (E). Scale bars: 20 μm. Green = anti–ZO-1, red = anti-CD46, blue = DAPI.
