Figure 2.
Culture of CB CD34+ cells with OAC1 resulted in increased number of CFUs. (a) CFU numbers in 1000 cells of Day 0 uncultured CD34+ cells and the progeny of an equivalent number of CD34+ cells that were expanded in the presence of vehicle control or OAC1 for 4 days. n=3, * indicates p<0.05, ** indicates p<0.01. (b) CFU numbers in the progeny of 1000 CD34+ cells that were expanded in the presence of vehicle control or OAC1 for 4 days with different doses of cytokines. Full dose: 100ng/mL SCF, 100ng/mL TPO and 100ng/mL Flt3L; 1/2 dose: 50ng/mL SCF, 50ng/mL TPO and 50ng/mL Flt3L; 1/4 dose: 25ng/mL SCF, 25ng/mL TPO and 25ng/mL Flt3L; 1/8 dose: 12.5ng/mL SCF, 12.5ng/mL TPO and 12.5ng/mL Flt3L. * indicates p<0.05, ** indicates p<0.01, N.S. indicates p>0.05. (c) CFU numbers in the progeny of 1000 CD34+ cells that were expanded in the presence of vehicle control or OAC1 for 4 days and different doses of cytokines were only employed during CFU assay. Full dose: 1 U/mL erythropoietin, 50 ng/mL SCF, 10 ng/mL IL-3, and 10 ng/mL GM-CSF; 1/2 dose: 0.5 U/mL erythropoietin, 25 ng/mL SCF, 5 ng/mL IL-3, and 5 ng/mL GM-CSF; 1/4 dose: 0.25 U/mL erythropoietin, 12.5 ng/mL SCF, 2.5 ng/mL IL-3, and 2.5 ng/mL GM-CSF; 1/8 dose: 0.125 U/mL erythropoietin, 6.25 ng/mL SCF, 1.25 ng/mL IL-3, and 1.25 ng/mL GM-CSF. * indicates p<0.05, ** indicates p<0.01, N.S. indicates p>0.05. Data represent the mean ± s.d.