Skip to main content
. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Theriogenology. 2015 Sep 3;85(3):376–383. doi: 10.1016/j.theriogenology.2015.08.015

Fig. 5. Northern Blot Analysis for Transcription of Vitellogenin mRNA in Livers of Roosters Treated With Estrogen, Test SERMs or combinations of estrogen and Test SERMs.

Fig. 5

The figure shows a chemiluminograph of a Northern blot of liver cytosolic extracts from roosters who received treatment with the vehicle (lane 1, panels A, B, C, & D), estrogen (lane 2, panels A, B, C, & D), resveratrol (lane 3, panels A, & B), genistein (lane 4, panels A, & B), catechin (lane 5, panels A, & B), tamoxifen (lane 6, panels A & B), pterostilbene (lane 3, panels C & D), raloxifene (lane 4, panels C & D), clomiphene (lane 5, panels C & D). Panels A and C were probed with biotinylated antisense chicken VG DNA oligonucleotide probe and panels B and D were probed with biotinylated antisense chicken β-actin DNA oligonucleotide probe. Panel E shows the antiestrogenic effect of the SERMs on VG gene expression and the relative electrophoretic gel mobility of the VG mRNA in relation to β-actin mRNA. Lanes 1-5 in panel E correspond to whole cell RNA from livers of roosters treated with the vehicle (lane 1), estrogen (lane 2), estrogen + resveratrol (lane 3), estrogen + genistein (lane 4), and estrogen + catechin (lane 5).