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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Theriogenology. 2015 Sep 3;85(3):376–383. doi: 10.1016/j.theriogenology.2015.08.015

Fig. 6. RNase T1 protection assay.

Fig. 6

Figure shows a chemiluminograph of 8M urea-6% polyacrylamide gel of RNase protection assay performed to detect apo II mRNA. Lane 1 shows the in vitro transcribed biotinylated antisense probe of upstream 3’-UTR of apo II mRNA that has irrelevant 5’ and 3’ overhangs, in the absence of RNase T1 digestion. Lanes 2-8, show RNase protection assays of whole cell RNA from livers of roosters treated with estrogen, resveratrol, genistein, catechin, tamoxifen, clomiphene and the vehicle, respectively. The band in lane 2 corresponds to the nuclease-protected biotinylated antisense probe of upstream 3’UTR of apo II mRNA.