Table 4.
Soil samples | Analysis of microbial diversity1) |
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---|---|---|---|---|---|---|---|---|---|
Analysis by PCR-DGGE method |
Analysis by BIOLOG method |
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Bacterial diversity |
Fungal diversity |
Shannon index (H′) | Substrate evenness (E) | Simpson's index (Ds) | |||||
Shannon index (H′) | Substrate evenness (E) | Simpson's index (Ds) | Shannon index (H′) | Substrate evenness (E) | Simpson's index (Ds) | ||||
R0 | 3.82 ± 0.009a,b | 0.98 ± 0.001b | 0.98 ± 0.001a,b | 2.63 ± 0.009c | 0.99 ± 0.000a,b | 0.94 ± 0.001a,b | 3.06 ± 0.02c | 0.98 ± 0.004b,c | 0.927 ± 0.012b |
R1 | 3.84 ± 0.009a | 0.97 ± 0.000d | 0.98 ± 0.000a,b | 2.27 ± 0.006e | 0.99 ± 0.000e | 0.93 ± 0.002a,b | 2.89 ± 0.02d | 0.99 ± 0.008a,b | 0.928 ± 0.002b |
R2 | 3.80 ± 0.007b | 0.96 ± 0.000f | 0.97 ± 0.000d | 2.61 ± 0.012c | 0.99 ± 0.000c,d | 0.94 ± 0.001d | 2.92 ± 0.03d | 1.00 ± 0.012a | 0.926 ± 0.003b |
R4 | 3.62 ± 0.015e | 0.97 ± 0.000e | 0.98 ± 0.001b | 2.68 ± 0.009b | 0.99 ± 0.001d,e | 0.94 ± 0.002b | 3.15 ± 0.01b | 0.98 ± 0.004b | 0.951 ± 0.001a |
R2 + 2 | 3.73 ± 0.015c | 0.97 ± 0.001c | 0.97 ± 0.000c | 2.36 ± 0.006d | 0.99 ± 0.001e | 0.93 ± 0.000c | 3.21 ± 0.01a | 0.96 ± 0.003c | 0.954 ± 0.001a |
R3 + 3 | 3.69 ± 0.009d | 0.97 ± 0.001d | 0.97 ± 0.000c | 2.37 ± 0.009d | 0.99 ± 0.001c | 0.94 ± 0.001c | 3.13 ± 0.01b | 0.98 ± 0.003b,c | 0.949 ± 0.001a |
RL-07 | 3.68 ± 0.009d | 0.98 ± 0.000a | 0.97 ± 0.001c | 2.91 ± 0.003a | 0.10 ± 0.001a | 0.95 ± 0.001a | 3.16 ± 0.02b | 0.98 ± 0.005b,c | 0.949 ± 0.001a |
RL-03 | 3.81 ± 0.003a,b | 0.99 ± 0.001a | 0.98 ± 0.000a | 2.92 ± 0.009b | 0.99 ± 0.001b | 0.94 ± 0.001c | 3.25 ± 0.01a | 0.97 ± 0.002b,c | 0.957 ± 0.001a |
LSR; PCR-DGGE, polymerase chain reaction and denaturing gradient gel electrophoresis
The letters indicate tested with Shortest Significant ranges (SSR) at p = 0.05 of different treatments. Different letters indicate a significant difference at p < 0.05 level