Fig. 3.

R9-caPep interferes with replication fork extension. Synchronized SK-N-BE(2)c cells were sequentially treated with two nucleotide analogs, CldU and IdU, before and after R9-caPep treatment. Cells sequentially treated with the same two nucleotide analogs without R9-caPep treatment were used as controls. The incorporation of these two nucleotides, representing replication fork extension before and after R9-caPep treatment respectively, was visualized by a fluorophore-coupled monoclonal antibody specific to CldU (green) or IdU (red). a) Shown are representative images of the labeled DNA strands from cells treated with or without R9-caPep. b) The lengths of IdU (red bars) and CldU (green bars) incorporated DNA segments measured from more than 30 independent DNA strands from cells treated with or without R9-caPep were averaged and graphed ± S.D.