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. 2016 Jan 7;3:70. doi: 10.3389/fchem.2015.00070

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Copyright © 2016 Nuriel, Whitehouse, Ma, Mercer, Brown and Gross.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of tryptophan nitration sites in peroxynitrite-treated rat brain homogenate and tyrosine nitration sites in untreated rat brain homogenate. The labeled MS/MS spectra from five nitrated peptides. The identities of each of the two tryptophan nitrated peptides discovered in the 1 mM ONOO⁻-treated brain homogenates (A,B) and each of the three tyrosine nitrated peptides discovered in the untreated rat brain homogenates (C–E) were manually validated using their MS/MS fragmentation data. Dimethylated lysines and carbamidomethylated cysteines are represented by under case letters, and nitration sites are represented by colored letters.