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. 2016 Jan 7;6:1509. doi: 10.3389/fmicb.2015.01509

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Copyright © 2016 Caroline, Kujawa, Oury, Reed and Hartman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Virus dissemination over time. Virus was measured in the indicated tissue by plaque assay (solid squares; pfu/g tissue) or taqman RT-PCR (open circles; pfu equivalents/g tissue). Horizontal line on each graph represents the limit of detection of the plaque assay (50 pfu). The gray shaded box on all graphs represents the window of clinical disease (5–7 dpi). For (A–C,F), the M on the x-axis stands for ‘moribund’ and represents historical samples from previous Lewis rat experiments (correspond to 7–8 dpi). Tissue samples were taken at the time the rat was moribund and are included here for comparison purposes. These samples are not available for (D) and (E).