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. 2016 Jan 7;6:1458. doi: 10.3389/fmicb.2015.01458

FIGURE 6.

FIGURE 6

Entry and growth of M. marinum inside macrophages. The entry of M. marinum wild-type, Δ2318, Δ2319, Δ2318::2318, and Δ2319::2319 strains into macrophages. The bacteria numbers inside macrophage cells on day 0 (3 h post infection) at MOI = 10 were determined. Entry was represented as the percentage of entry CFU vs. initial CFU of three independent experiments. No significant differences were noted among the wild-type strain, the deletion mutants (Δ2318 and Δ2319), and the complementation strains (Δ2318::2318 and Δ2319::2319) into J774a.1 (A) but the recovered bacteria number of two deletion mutants were significantly higher than wild-type strain into THP-1 (D). Data from three independent experiments are presented as the mean ± SEM of the % of initial CFU. ∗∗p < 0.001. Growth kinetics of the M. marinum wild-type, Δ2318, Δ2319, Δ2318::2318, and Δ2319::2319 strain inside macrophages. The bacteria numbers inside J774a.1 (B,C) or THP-1 (E) cells on days 0 (3 h), 2, 4, 6, and 8 days at MOI = 1 were determined. Data from three independent experiments are presented as the mean ± SEM of the fold of initial entering bacteria number.