Skip to main content
. 2015 Oct 16;5(1):5–18. doi: 10.1016/j.molmet.2015.10.001

Figure 3.

Figure 3

Enforced glycogen deposition in human adipocytes stimulates leptin secretion through an autophagy-dependent mechanism. Differentiated SGBS adipocytes (n = 4–5) were transduced with Ad-PTG or control Ad-GFP and analyzed 48 h post-infection. (A–B) Total cell extracts from transduced differentiated SGBS cells were subjected to immunoblotting with the indicated antibodies. GAPDH was used as a loading control. (C) Adipocytes were treated or not with 5 mM MHY1485. Total cell extracts were subjected to immunoblotting with antibodies against phosphorylated ULK1 (Ser758), L3CBII and PTG. GAPDH was used as a loading control. (D) Transduced SGBS adipocytes were treated or not with 5 mM 3-MA. Total cell extracts were subjected to immunoblotting with antibodies against GABARAPL1 and PTG. GAPDH was used as a loading control. (E) Immunofluorescence detection of glycogen (red). Nuclei were stained with DAPI (blue). (F) Leptin expression and secretion was analyzed by RT-qPCR and ELISA, respectively. *p < 0.01 vs. control cells. Statistical significance was tested with unpaired Student's t test followed by the protected least-significance different test.