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. 2015 Nov 6;5(1):34–46. doi: 10.1016/j.molmet.2015.10.004

Figure 2.

Figure 2

SIK1-knockout mice are protected from insulin resistance in obesity. A) GTT of male global SIK1-KO mice after 16–18 weeks of high fat diet feeding (mean ± stdev; n = 6–8 per genotype among 2 cohorts; 2-way repeated measures ANOVA p < 0.0001; Bonferroni post-tests: *, p < 0.05; ***, p < 0.001 vs WT at indicated time points). B) Area under curve analysis of GTT in panel A (mean ± stdev; n = 6–8 per genotype; *, p < 0.05). C) Plasma insulin in HFD fed male SIK1-KO mice after overnight fast and 15 min after glucose injection (mean ± stdev; n = 11–12 per genotype among 2 cohorts; *, p < 0.05). D) ITT of male HFD SIK1-KO mice (mean ± stdev; n = 4–5 per genotype; 2-way repeated measures ANOVA p = 0.06; Bonferroni post-test: ***, p < 0.001 vs WT at indicated time points). E) Western blots of phosphorylated and total AKT in quadriceps muscle from male HFD-fed SIK1-WT and KO animals euthanized ad libitum or after 6 h fast and IP insulin (15 min). Each lane represents one animal (n = 4 per condition). See Supplementary Figure S3F for densitometry. F) Rate of endogenous glucose appearance (EndoRa) in fasted HFD male SIK1-WT and SIK1-KO mice 60 min after [3-3H]-glucose tracer infusion (mean ± stdev; n = 4–5 per genotype). G) Glucose infusion rate (GIR) at the final time point of hyperinsulinemic euglycemic clamp in dynamic steady state (mean ± stdev; n = 4–5 per genotype; **, p < 0.01). See Supplementary Figure S3M,N for time courses. H) Insulin-stimulated biodistribution of 18F-fluorodeoxyglucose in male HFD fed mice (mean ± stdev; n = 4–5 per genotype; *, p < 0.05 vs WT).