Table 2.
Effects of G-Ro on platelet aggregation, [Ca2+]i mobilization, and cyclic adenosine monophosphate production in thrombin-induced human platelet activation
| Aggregation (%) | Δ (%) | cAMP (pmol/108 platelets) |
Δ (%) | Ca2+ (nM) | Δ (%) | |
|---|---|---|---|---|---|---|
| Basal (control) | - | 4.8 ± 0.3 | — | 102.4 ± 0.5 | — | |
| Thrombin (0.05 U/mL) | 86.7 ± 1.5 | — | 4.6 ± 0.1 | 0 | 655.7 ± 38.7 | +540.3 2) |
| G-Ro (200 μM) + Thrombin (0.05 U/mL) | 41.7 ± 2.5* | −52.0 1) | 8.7 ± 1.0* | +89.1 1) | 149.0 ± 3.8* | −77.3 1) |
The data are expressed as the mean ± standard deviation (n = 4)
* p < 0.05 versus the thrombin-stimulated platelets
cAMP = cyclic adenosine monophosphate
1)
Δ (%) = [Thrombin – (G-Ro + Thrombin) / Thrombin × 100
2)
(Basal – Thrombin)/Basal × 100