Fig. 3.

Acute gintonin bath application has no effect on synaptic plasticity in the CA3-CA1 synapses. (A) Theta burst stimulations (TBS, 20 bursts of 4 pulses at 100 Hz, repeated twice in 10 s) were given at Shaffer collateral pathway 1 h after gintonin or vehicle incubation (3 μg/mL). (B, C) Upon 1 h gintonin incubation, TBS-induced fold changes in both rise slope (10–90%) and amplitude of the stimulated pathway (stim. path.) remained comparable with vehicle group (42% vs. 42% for rise slope, p > 0.9; 70% vs. 50%, for amplitude, p > 0.4, vehicle (v) vs. gintonin (g) group, respectively, con.path.: control pathway, n = 5–7). The gray trace represents an averaged field excitatory postsynaptic potentials (fEPSP) during baseline and the overlaid black trace represents an averaged fEPSP response 50–60 min after TBS stimulation (scale bar: 20 ms and 0.2 mV). Error bars represent standard error of the mean.