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. 2016 Jan 7;6:1203. doi: 10.3389/fpls.2015.01203

FIGURE 3.

FIGURE 3

The salt induced EIN3 protein and transcript levels were modulated by NO release. (A) High salt promotes EIN3 protein accumulation. 5-day-old seedlings of Col-0, ein3-1eil1-1, and nia1-1nia2-5 were treated with 200 mM NaCl for 3 and 6 h. Protein was extracted and subjected to immunoblots using anti-EIN3 antibody. The non-specific band was used as a loading control. (B) EIN3 transcript analysis in wild type. 5-day-old seedlings were treated with 200 mM NaCl for 3 and 6 h. (C) EIN3 transcript analysis in nia1-1nia2-5. 5-day-old seedlings were treated with 200 mM NaCl for 3 and 6 h. (D) cPTIO impairs high salt induced EIN3 accumulation in wild type Col-0. 5-day-old seedlings were treated with 200 mM NaCl alone or plus 200 μM cPTIO for 6 h. The non-specific band was used as a loading control. Data are mean values (SE) of three independent experiments. Within each set of experiments, bars with different letters were significantly different at the 0.05 level (Duncan’s test).