Figure 1. Synthesis and cellular localization of caged AA derivatives.

(a) Synthesis of the sulfonated coumarin alcohol 1 and caged FAs. (b) Schematic representation of plasma membrane-specific photoactivation of caged signalling lipids. (c) Cellular localization of AA derivatives 6 (top), 7 (middle) and 5 (bottom) with different coumarin cages (coumarin—yellow, plasma membrane marker—magenta, nuclear marker—blue). Images were acquired using identical acquisition settings directly after removing loading solutions. All images are presented at the same magnification; the scale bar indicates 20 μm.