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. 2015 Dec 21;6:10077. doi: 10.1038/ncomms10077

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Nuclear lysates from MCF7-Ctrl and Six1 cells (a) treated with etoposide (10 μM) for 2 h were subsequently treated with cycloheximide (20 μM) and collected after indicated periods of time. Right: p53 half-life was calculated by quantification of western blots using Image Lab software. (b) MCF7-Six1 and Ctrl cell lines treated with etoposide (10 μM) and MG132 (25 μM) for indicated periods of time. Membranes were probed for Cyclin-D1 to demonstrate that the MG132 used was effective.