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. 2015 Nov 14;5(1):57–65. doi: 10.1016/j.molmet.2015.11.001

Figure 2.

Figure 2

Ebf2 is required for beiging. (A) Relative EBF2 protein levels from WT primary ingWAT stromal vascular cells plus or minus rosi treatment as assayed by western blot. (B) Relative Ebf2 mRNA levels from WT or Ebf2 KO primary ingWAT stromal vascular cells before and after rosi treatment as measured by RT-qPCR. (C) Oil Red O staining of differentiated and rosi treated WT or Ebf2 KO primary ingWAT stromal vascular cells. (D–G) Relative mRNA levels from WT or Ebf2 KO primary ingWAT stromal vascular cells plus or minus rosi treatment as measured by RT-qPCR. Levels of (D) the general adipose genes Pparϒ and Fabp4, (E) the brown selective genes Ucp1 and Cidea, (F) the brown selective genes Pgc1α, Pparα and Prdm16, and (G) the mitochondrial genes Cox5b, Cox7a1, Cox8b. (H) Western blot to measure protein levels of UCP1 in differentiated and rosi treated WT or Ebf2 knockout primary ingWAT stromal vascular cells. NS = non-specific band loading control. (I) Relative Ucp1 and Pgc1α mRNA levels from WT or Ebf2 knockout primary ingWAT stromal vascular cells before and after Iso treatment as measured by RT-qPCR. P-values are represented with asterisks (*, p-value ≤ .05; **, p-value ≤ .01; ***, p-value ≤ .001) and error bars represent standard deviation.