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. 2015 Dec 16;6:10172. doi: 10.1038/ncomms10172

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Coomassie-stained gel showing Hb, Hb:Hp and albumin control (BSA) beads pulling down KdHpuA (lanes 1–3), NgHpuA (lanes 4–6) and EcHpuA (lanes 7–9) proteins. Binding reactions were carried out in 500 ml solutions containing 3 μM HpuA. Input samples of all proteins used are shown in lanes 10–15.