Figure 7. SARD1 and CBP60g modulate the expression of genes encoding negative regulators of plant defence.

(a,b) Recruitment of SARD1-HA (a) and CBP60g-HA (b) to the promoter regions of PUB13, WRKY40, WRKY60, NUDT6, NUDT7, MLO2, BON1, BAP1 and BAP2 following P.s.m. ES4326 infection as determined by ChIP-PCR. ChIP was performed as described in Fig. 2. Real-time PCR was carried out using gene-specific primers. ChIP results are presented as fold changes by dividing signals from ChIP with the anti-HA antibody by those of the IgG controls, which are set as one. Bars represent means±s.d. (n=3). (c) Induction of PUB13, WRKY40, WRKY60, NUDT6, NUDT7, MLO2, BON1, BAP1 and BAP2 genes in wild type and sard1-1 cpb60g-1 by P.s.m. ES4326. Samples were collected 12 h after inoculation with P.s.m. ES4326 (OD₆₀₀=0.001) or 10 mM MgCl₂ (mock). Expression levels of the genes were normalized with ACTIN1. Bars represent means±s.d. (n=3).