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. 2015 Dec 18;6:10159. doi: 10.1038/ncomms10159

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(a) Reporter constructs used in the promoter activity assay. NOS101, a basal promoter of the nopaline synthase gene (−101 to +4); ICS1-56, a 56 bp fragment from the ChIP-Seq peak region in ICS1 promoter; ICS1-56m and ICS1-56m2, mutant versions of ICS1-56 with mutations (underlined) in the GAAATTT or GAAATT motif. The GAAATTT and GAAATT sequences are bolded. (b) Luciferase activities in protoplasts transformed with individual reporter constructs. (c) Luciferase activities in protoplasts transformed with individual reporter constructs together with a 35S-SARD1 plasmid. A 35S-Renilla Luciferase construct was included in all assays as an internal transformation efficiency control. The activities of luciferase were normalized with the expression of the Renilla luciferase and compared with the value obtained from protoplasts transfected with NOS101-LUC construct, which was set as 1. The error bars represent means±s.d. from three biological replicates.