Figure 5. Interaction with SRF recruits YAP to MaSC signature-gene promoters.
(a) ChIP–qPCR analyses of the indicated transcriptional regulators (n=3 experiments). (b) Luciferase assay of the IL6 promoter CArG box (n=3 experiments). (c) EMSA assay of the IL6 promoter CArG box using 293T extracts expressing indicated genes and GST-tagged proteins. (d) ChIP–qPCR analyses of the indicated cells (n=3 experiments). (e) ChIP–qPCR analyses of the TEAD-binding boxes of CTGF/CYR61 promoters and CArG boxes of IL6/DLL1 promoters in MCF-10A cells expressing the indicated mutant YAP (n=3 experiments). YAP 5SA mutants are used to maximize chromatin binding. Data are presented as means±s.e.m. (*P<0.05, **P<0.01, ***P<0.001, NS, not significant (P>0.05), Student's t-test used in all analyses).