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. 2015 Dec 16;6:10186. doi: 10.1038/ncomms10186

Figure 9. YAP is a BLBC-specific inducer of stemness.

Figure 9

(a) A panel of breast cancer cell lines was analysed using semiquantitative RT–PCR and western blot analysis using the indicated antibodies. (b) Statistical analyses of CD44 and CD24 antigen expression in basal and luminal cell lines overexpressing YAP, determined by FACS (n=3 experiments). (c) Mammosphere assay of cells generated in b (n=3 experiments). (d) Scatter plot of IL6 and CTGF expression versus YAP+TAZ expression level in luminal cancer and BLBC. Note that IL6 and YAP/TAZ expression levels are not significantly correlated in luminal cancer (n>100 for each subtype; correlation tested using Pearson's correlation coefficient, r). (e) RT-qPCR analyses of IL6 mRNA expression for the indicated luminal and basal cancer cell lines overexpressing YAP (n=3 replicates). (f) Kaplan–Meier curves for luminal-type breast cancer patients and BLBC patients with different levels of YAP/TAZ expression (log-rank test). Refer to Supplementary Fig. 12f for survival curves for other breast cancer subtypes. (g) Molecular model of BLBC-specific YAP induction of MaSC properties. Data are presented as means±s.e.m. (*P<0.05, **P<0.01, ***P<0.001; NS, not significant (P>0.05), Student's t-test used in all analyses).