Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 7;6:18934. doi: 10.1038/srep18934

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) The 2:1 stoichiometry of the NEMO-Ub₂ complex is supported by comparison of the SAXS experimental curves with theoretical curves back-calculated from either the 2:2 NEMO-Ub₂ co-crystal structure (PDB 2ZVO, 2:2) or a 2:1 complex, where one Ub₂ was removed from the crystal structure. (b) The calculated MWs in the table show that independent of the mixing ratio of NEMO_UBAN and Ub₂ a 2:1 complex is formed, and (c) the 2:1 ratio is further supported by the shape of the envelope of ab initio modeling with DAMMIF (shown in yellow). (d) NMR analysis of the NEMO_UBAN-linUb₂ interaction. ¹H,¹⁵N TROSY NMR spectrum of 100 μM ¹⁵N-labeled NEMO in the absence (black) or presence of Ub₂ (red) in a 2:1 (NEMO_UBAN:Ub₂) ratio. Amides, which disappear upon Ub₂ addition are indicated. (e) Mapping of the backbone amides, which disappear upon linUb₂ addition in panel d onto the NEMO_UBAN surface from the crystal structure (PDB 2ZVO).