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RP-HPLC on an Aquapore C8 RP300 (4.6 mm × 100 mm) column of fractions V (A), III (B), and IV (C) obtained by SE-chromatography on Sephacryl S400 (see Figure 3). For experimental details, see section ‘Materials and Methods.’ The 10.033-Da protein is shown by an arrow.
