Table 1.

Measured diffusion coefficients for DiD in the mitochondria-like membrane. Errors are the standard error of the mean (SEM), except for the value of D extracted from the MSD of the combined track, which was calculated by adjusting the fitting window, as explained in the text, and for that extracted from the distribution of displacements, in which case, the error is the standard deviation of the values obtained for different lag times.

Experimental Method	Analysis Method	D (μm2 · s−1)
Line-scanning FCS	Full images	3.4 ± 0.4
	Cropped images	3.2 ± 0.4
SPT	MSD of individual tracks	2.4 ± 0.3
	MSD of combined track	2.2 ± 0.4
	Distribution of displacements	1.6 ± 0.2