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. 2016 Jan 7;6:19008. doi: 10.1038/srep19008

Figure 8. Agonism on GPBAR1 by transactivation assay.

Figure 8

(A) HEK-293T cells were co-transfected with GPBAR1 and a reporter gene containing a cAMP responsive element in front of the luciferase gene. Twenty-four hour post transfection cells were stimulated with 7–21 (10 μM). Luciferase activity served as a measure of the rise in intracellular cAMP following activation of GPBAR1. TLCA (2, 10 μM) was used as a positive control. Results are expressed as mean ± standard error. *p < 0.05 versus not treated cells (NT); (B) Concentration-response curve of 9 and 21 on GPBAR1 in HEK-293T cells co-transfected with GPBAR1 and a reporter gene containing a cAMP responsive element in front of the luciferase gene (CRE). Twenty-four hour post transfection cells were stimulated with increasing concentrations of each agent: range from 100 nM to 50 μM. Results are expressed as mean ± standard error.