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. 2016 Jan 7;6:19035. doi: 10.1038/srep19035

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Copyright © 2016, Macmillan Publishers Limited

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(a) Cell and virion lysates were subjected to western blotting using antibodies specific for the HA tag and HIV-1 Gag proteins. The blot of the proteins present in the cell lysates was also probed with an anti-β-actin antibody. Data from one experiment representative of three independent experiments are shown. (b) Relative packaging efficiency. A1 packaging levels were quantified based on band intensities of HA-tagged A1 normalized to each p24 of the virions and shown graphically as relative packaging to human A1. The average (and standard deviation) of three experiments is indicated. p-values are shown in comparison to human A1. *p < 0.05, **p < 0.01, ***p < 0.001, NS, no statistical significance. (c) Correlation of packaging levels with HIV-1 infectivity. (d) Correlation of packaging levels with cytoplasmic localization.