Table 3. Two-way ANOVA tests analysing direct and combined effects of low pH and thermal-stress (+2 °C) on three coralline species: Neogoniolithon sp. (rhodolith), Amphiroa tribulus (articulated) and the CCA Lithothamnion sp. Pmax, maximum gross photosynthesis (μmol O2 cm−2 h−1); RL, Post-illumination Respiration (μmol O2 cm−2 h−1); and Gmax, maximum calcification rate (μmol CaCO3 cm−2 h−1).
Rodoliths |
F | P | Articulated |
F | P | CCA | MS | F | P | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|
df | MS | df | MS | df | ||||||||
Source of variation | ||||||||||||
Pmax | ||||||||||||
pH | 1 | 0.564 | 3.35 | 0.08 | 1 | 0.255 | 1.75 | 0.199 | 1 | <0.001 | 0.001 | 0.981 |
Temp. | 1 | 5.319 | 31.5 | <0.05* | 1 | 6.298 | 43.44 | <0.05* | 1 | 0.603 | 83.014 | <0.05* |
pH*temp. | 1 | 0.254 | 1.5 | 0.232 | 1 | 0.001 | 0.009 | 0.926 | 25 | 0.003 | 0.428 | 0.519 |
Error | 24 | 0.168 | 21 | 0.145 | 0.007 | |||||||
RL | ||||||||||||
pH | 1 | 0.07 | 1.804 | 0.192 | 1 | 0.012 | 0.677 | 0.419 | 1 | 0.005 | 1.522 | 0.229 |
Temp. | 1 | 1.173 | 30.393 | <0.05* | 1 | 1.478 | 84.274 | <0.05* | 1 | 0.112 | 36.563 | <0.05* |
pH*temp. | 1 | 0.005 | 0.132 | 0.72 | 1 | 0.028 | 1.619 | 0.216 | 1 | <0.001 | 0.023 | 0.882 |
Error | 24 | 0.039 | 21 | 0.018 | 25 | 0.003 | ||||||
Gmax | ||||||||||||
pH | 1 | 0.308 | 19.17 | <0.05* | 1 | 0.074 | 29.6 | <0.05* | 1 | 0.029 | 18.235 | <0.05* |
Temp. | 1 | 0.59 | 36.62 | <0.05* | 1 | 1.207 | 483.14 | <0.05* | 1 | 0.111 | 69.577 | <0.05* |
pH*temp. | 1 | 0.001 | 0.056 | 0.816 | 1 | 0.089 | 35.58 | <0.05* | 1 | <0.001 | 0.074 | 0.788 |
Error | 16 | 0.016 | 22 | 0.002 | 23 | 0.002 |