Table 1.

F.IX levels in the conditioned medium from murine C2C12 cells transduced with AAV-CMV-F.IX vectors at a range of multiplicity of infection

Multiplicity of Infection (MOI)	AAV-1, F.IX ng/mL per 48 h	AAV-2, F.IX ng/mL per 48 h	Fold difference
Undifferentiated C2C12 cells
1 000	3 ± 1	12 ± 2.6	4
5 000	19 ± 4	60 ± 13	3
20 000	29 ± 10	108 ± 37	3.7
40 000	36 ± 4.5	129 ± 40	3.5
Differentiated C2C12 cells
1 000	33 ± 10	107 ± 47	3.2
5 000	63 ± 29	126 ± 29.4	2
20 000	103 ± 12	208 ± 30	2
40 000	115 ± 8	247 ± 58	2
Fold range difference	3 to 10	2 to 15

Over prolonged periods and over a broad dose range, AAV-1– and AAV-6–based vectors result in higher levels of expression of a F.IX transgene compared with AAV-2 vectors in immunodeficient mice.