TABLE 1.
Primer sequences and RT-qPCR cycling conditionsa
| Target | Primer sequence (5′→3′) | PCR product length (bp) |
|---|---|---|
| P. vivax β-tubulin gene | F: TTC CCCA ACA ACAC CAA GTCC | 178 |
| R: TCC ATGC CCT CTCC TGT GTA | ||
| P. vivax aldolase gene | F: GAC AGT G CCA CC AT CCT TACC | 191 |
| R: CCT TCT CAA CAT TCT CCT TCT TTC C | ||
| pvcrt-o | F: TTA TCTG CAT CCCG TCA TCC | 159 |
| R: GTA TTTC GCT CTCA TTC TGTGC |
a
PCR cycling conditions consisted of 95°C for 5 min followed by 40 cycles of denaturation at 95°C for 60 s, annealing at 60°C for 60 s, and extension at 72°C for 60 s.