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. 2015 Dec 31;60(1):24–35. doi: 10.1128/AAC.01509-15

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Copyright © 2015 Aung et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 13 — Combination treatment of Gatifloxacin (10× MIC) and DNase (12.5 μg/ml) on M. chelonae biofilm. Confocal images of M. chelonae biofilm after treatment with gatifloxacin at 10× MIC (A) or AM-228 at 10× MIC and DNase at 12.5 μg/ml (B) are shown. Green color represents live biomass, whereas red is dead biomass. Histograms show comparison of live/dead ratios and viable bacteria (CFU) after treatment of M. chelonae biofilm. Scale bar, 50 μm. The values shown are means ± SDs from one representative experiment (five images); three independent experiments were done. Combination treatment was more effective than individual treatment. Live/dead ratios and CFU were compared using the Mann-Whitney U test for the combination versus gatifloxacin (P = 0.002 for biovolume and P = 0.021 for CFU). *, P ≤ 0.05 (statistically significant).