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Penisin structure determination via NMR. (A) 1H NMR spectrum of the A3 peptide in water. (B) 1H NMR spectrum of the A3 peptide in TFE. The amide hydrogens region is highlighted (δ 8.5 to 8.0). All spectra were obtained at pH 7.0, in 10 mM Tris-HCl buffer at 37°C.
