TABLE 3.
Activity of azoles in S. cerevisiae containing C. albicans ERG11 alleles
| S. cerevisiae | Resistance mechanism/genotype | MIC (μg/ml [fold differencea]) |
||||
|---|---|---|---|---|---|---|
| Fluconazole | Itraconazole | Posaconazole | Voriconazole | Isavuconazole | ||
| DSY595b | YKKB13 and C. albicans ERG11 | 4 (1) | 0.25 (1) | 0.5 (1) | 0.125 (1) | 0.125 (1) |
| DSY1109 | YKKB13 and C. albicans ERG11 (G129A) | 4 (1) | 0.25 (1) | 0.5 (1) | 0.0156 (1) | 0.125 (1) |
| DSY949 | YKKB13 and C. albicans ERG11 (S405F) | 8 (2) | 0.25 (1) | 0.5 (1) | 0.125 (1) | 0.25 (2) |
| DSY952 | YKKB13 and C. albicans ERG11 (Y132H) | 8 (2) | 0.5 (2) | 0.25 (1) | 0.25 (2) | 0.5 (4) |
| DSY950 | YKKB13 and C. albicans ERG11 (G464S) | 8 (2) | 0.25 (1) | 0.5 (1) | 0.125 (1) | 0.125 (1) |
| DSY951 | YKKB13 and C. albicans ERG11 (R467K) | 8 (2) | 0.25 (1) | 0.5 (1) | 0.125 (1) | 0.25 (2) |
| DSY963 | YKKB13 and C. albicans ERG11 (S405F/Y132H) | 128 (32) | 1 (4) | 0.5 (1) | 2 (16) | 1 (8) |
| DSY965 | YKKB13 and C. albicans ERG11 (G464S/Y132H) | 32 (8) | 0.5 (2) | 0.5 (1) | 2 (16) | 1 (8) |
| DSY1107 | YKKB13 and C. albicans ERG11 (G464S/R467K) | 32 (8) | 0.25 (1) | 0.5 (1) | 0.25 (2) | 8 (4) |
| DSY1108 | YKKB13 and C. albicans ERG11 (G464S/G129A) | 15 (4) | 0.25 (1) | 0.5 (1) | 0.125 (1) | 0.25 (2) |
| DSY612 | YKKB13 and YEp51c | 1 (0.25) | 0.0625 (0.25) | 0.25 (0.5) | 0.0078 (0.0625) | 0.0625 (0.5) |
a
Fold difference is relative to the level for DSY595 for each azole.
b
Baseline strain.
c
Yeast replicating vector (parent vector) into which ERG11 alleles were cloned.