FIG 3.

Morphological analysis of E. coli membrane with CA-MA and CMA3 peptides. (A) Localization of TAMRA-labeled CA-MA and CMA3 in E. coli. The cells were incubated for 10 min with the MIC of TAMRA–CA-MA (top) or TAMRA-CMA3 (bottom) and then examined using a confocal laser-scanning microscope. The scale bar is 5 μm. DIC, differential interference contrast. (B) Morphological analysis of E. coli cells in the absence and presence of CA-MA and CMA3 using SEM. CA-MA and CMA3 were incubated with E. coli cells at the MIC in 10 mM sodium phosphate buffer (containing 10% medium). The scale bar is 1.0 μm.