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. 2015 Dec 31;60(1):495–506. doi: 10.1128/AAC.01998-15

FIG 4.

FIG 4

CMA3 binding to E. coli LPS, as evaluated using CD (A), TAMRA-labeled peptide (B), and a limulus amebocyte lysate chromogenic endotoxin quantitation kit (C). (A) Far-UV CD spectra confirming that structural changes are induced upon mixing 30 μM CMA3 with 30 mM LPS. (B) Relative binding of the TAMRA-labeled CMA3 in the presence of 25 μg/ml LPS at 37°C (fluorescence spectrometry). Mean values are presented; n = 3. Error bars indicate SD (P < 0.005). (C) LPS neutralization by CMA3 determined using an endotoxin quantitation kit. Mean values are presented; n = 3. Error bars indicate SD (P < 0.005). (D) Diagram of CMA3 binding to and neutralizing LPS in the bacterial membrane. The peptide structures were calculated by using PEP-FOLD server (http://bioserv.rpbs.univ-paris-diderot.fr/services/PEP-FOLD/).