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. 2015 Dec 31;60(1):544–553. doi: 10.1128/AAC.01650-15

FIG 3.

FIG 3

Induction of PhoPQ- and PmrAB-controlled genes under acidic pH conditions. (A) Influence of limiting Mg2+ (125 μM) on the expression of oprH-lux, PA3553::lux, and PA4774::lux compared to that in BM2 defined medium at neutral pH 7 with 2 mM Mg2+. (B) Influence of acidic pH (5.5) on the expression of oprH-lux, PA3553::lux, and PA4774::lux compared to that in BM2 defined medium at neutral pH 7. (C) Influence of mildly acidic pH (5.5) in rich LB medium on the expression of oprH-lux, PA3553::lux, and PA4774::lux. (D) Effects of various pH values ranging between 5.5 and 7 on the expression of PA3553::lux and PA4774::lux. All values shown are the averages and standard deviations from 4 replicates. Gene expression is shown at ∼7 h of growth under the respective conditions.