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DNA- and pH-induced aminoglycoside resistance in P. aeruginosa PAO1 is blocked by neutralizing the pH
| Medium condition | pH | MIC (μg/ml) |
|
|---|---|---|---|
| Gm | Tm | ||
| eDNA | |||
| LB + DNA (0.2%) | 5.75 | 16 | 16 |
| LB | 7 | 4 | 2 |
| LB + DNA + 10 μM Mg2+ | 5.76 | 8 | 8 |
| LB + DNA + 10 mM Tris | 7.02 | 8 | 4 |
| LB + DNA + 10 μM Mg2+ + 10 mM Tris | 7 | 2 | 2 |
| LB + DNA + l-Arg (0.4%) | 8.25 | 4 | 2 |
| LB + DNA + 10 mM NaHCO3 | 7.4 | 2 | 1 |
| Acid pH | |||
| LB (pH 5.5) | 5.5 | 8 | 8 |
| LB | 7 | 1 | 1 |
| LB (pH 5.5) + l-Arg (0.4%) | 8.25 | 2 | 1 |
