Plasmid analysis. (A) Lane 1, Kp2; lane 2, transconjugant (Tc) Kp2; lane 3, Kp4; lane 4, Tc Kp4 B; lane 5, pIP173 (126 kb); lane 6, RP4 (55 kb) and pCFF04 (85 kb); lane 7, Ec7; lane 8, Tc Ec7; lane 9, Kp8; lane 10, Tc Kp8. (B) Lane 1, pIP173 (126 kb); lane 2, RP4 (55 kb) and pCFF04 (85 kb); lane 3, Kp10; lane 4, transformant (Tf) Kp10.