FIG 2.

Analysis of LEE gene expression in the presence of ketolides by using GFP fusions. Individual promoters of each LEE operon were fused with GFP and transformed into E. coli O157:H7 strain ZAP198 (Table 1). Plasmid pAJR145 is a control fusion in which the rpsM promoter was fused with gfp (rpsM::gfp) and transformed into ZAP198. The fluorescence and OD₆₀₀ were determined every hour. ZAP198 transformed with a promoter-less GFP plasmid (pKC26) was used as a background control. Lines represent the means of three biological repeats ± 1 SD. The results of one representative experiment out of at least three that were performed with similar results are shown. P < 0.005, ANOVA; ***, P < 0.001, Newman-Keuls multiple-comparison test, compared to the results for the control. OD₆₀₀, optical density at 600 nm; AU, arbitrary units; TEL, telithromycin; SOL, solithromycin.