TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| ZAP193 | Stx negative, NCTC 12900 | 33 |
| ZAP198 | EHEC O157:H7 strain Walla 3 Nalr | 74 |
| ΔLEE2 | ZAP193 derivative with LEE2 deletion | This study |
| ZAP1004 | ZAP198 derivative with ler deletion | 75 |
| TUV93-0 | EHEC O157:H7 strain EDL933 stx negative derivative | 32 |
| Plasmids | ||
| pKC26 | Promoter-less GFP plasmid | 76 |
| pKC26sulA | GFP fusion plasmid with promoter from sulA | This study |
| pDWLEE1 | GFP fusion plasmid with promoter from LEE1 operon | This study |
| pDWLEE2 | GFP fusion plasmid with promoter from LEE2 operon | This study |
| pDWLEE3 | GFP fusion plasmid with promoter from LEE3 operon | This study |
| pDW6 | GFP fusion plasmid with promoter from LEE4 operon | 79 |
| pDWLEE5 | GFP fusion plasmid with promoter from LEE5 operon | This study |
| pAJR145 | pACYC rpsM::gfp | 34 |
| pAJR71 | pACYC LEE1::gfp | 33 |
| pWSK29 | Low-copy-no. vector, Ampr | 77 |