Fig. 4.

OPA1 ^+/−-iPSCs failed to differentiate into RGCs with culture medium supplemented with 10 % FBS and DAPT. Neurospheres were plated onto PLO/L-coated plates and cultured in hESC medium containing 10 μM DAPT and 10 % FBS for 7 days before fixation with 4 % PFA on day 24. Cells were stained with TUJ1 (red), BRN3a (green, a) and ISLET-1 (green, b) antibodies. Scale bars = 20 μM